New answers tagged neuroimaging
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I've used latex microspheres in the past. These not only enter cells but also move retrograde (backward) through axons, and are sized up 0.02 to 0.2 microns; it seems like the smaller beads in the range of 0.05 microns are taken up better (or at least transported better) than the larger ones. You can see the individual spheres clearly on a microscope in ...
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Yes, for example there is the, uhh, creatively named "glue sniffer": GluSnFRs that responds to glutamate by fluorescing, and a couple other varieties as well (also mentioned on that Wikipedia page).
The concept is similar to the calcium-sensitive dyes: couple a motif that binds to the target of interest and causes a conformational change with a ...
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When using a genetically encoded fluorescent dye like GCaMPs, the calcium-sensitive proteins are produced in and located inside the cell. That's where they have to be because they are measuring the calcium concentration inside the cell.
When using an injected dye like Oregon Green BAPTA, it's common to use an AM ester form of the dye (for example see this ...
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