I am tracking eye movements in mice passively viewing naturalistic stimuli or experimental stimuli meant to elicit reflexive eye movements. I would like to define a transformation function that translates displacement in pixels (x, y) to degrees of visual angle (azimuth, elevation). Here is an example image:
I have seen this paper referenced several times in other papers that track eye movements in mice, but it seems like the position of my cameras relative to the eye is different than what is shown in the paper and I worry this will affect the validity of the transformation.
I have the ability to track any feature of the eye that is useful. Right now I am tracking the center of the pupil, the corners of the eye, and the center of the top and bottom eyelid.
Here is a description of the camera's position relative to the animal. The solid black object represents the animal (A=anterior, P=posterior, D=dorsal, V=ventral). The angle from each orientation represents the angle from the animal to the camera.